Do Mushroom spores die when frozen?
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                            A spore is almost like a bacteria or a virus...they go dormant(chemical changes occur due to environment)... If they already germinated while in a liquid solution and THEN froze... yes, you will have a problem...they will be useless. A frozen syringe can expand causing damage to the actual syringe. 
                    
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                                   Put a drop of water on the top of the cap to help release the spores. Cover the cap with a paper cup or glass and leave for 2-24 hours, depending on the humidity and the freshness of the mushroom. The spores will fall on the paper, foil or glass, making a spore print pattern.. 
                                
                            
                        
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                            There is a variety of ways to store your mushroom spore prints but the most common and simple method is to put the print in a ziplock bag, sealed and let it sit in the fridge. You can even simply keep them in file folder or any location that is clean, dry, and average temperature. 
                        
                    
                        
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                            Most yeasts and molds are heat-sensitive and destroyed by heat treatments at temperatures of 140-160°F (60-71°C). Some molds make heat-resistant spores, however, and can survive heat treatments in pickled vegetable products.. 
                        
                    
                        
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                            The spore print is the powdery deposit obtained by allowing spores of a fungal fruit body to fall onto a surface underneath. It is an important diagnostic character in most handbooks for identifying mushrooms. It shows the color of the mushroom spores if viewed en masse.. 
                        
                    
                        
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                            The spore print is the powdery deposit obtained by allowing spores of a fungal fruit body to fall onto a surface underneath. It is an important diagnostic character in most handbooks for identifying mushrooms. It shows the color of the mushroom spores if viewed en masse.. 
                        
                    
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